Optimize, standardize, automate and document your decalcification protocols
The DecalMATE system is a new tool that enables histotechs to “tailor” their fixation/decalcification process to the
specific characteristics of their bone tissue specimens.
Simple, automatic, safe.
For the first time, automatic protocols for the fixation/decalcification of bone tissues are available to modern laboratories to improve the diagnostic quality and the consistency of results with a faster turnaround time. The intuitive, user-friendly software guides the operator on the touch screen control terminal. The unit consists of a resistance-heating, temperature controlled process cavity, made of high chemical resistant techno polymers, with a built-in magnetic stirring device for temperature and concentration homogeneity.
After placing the rack in the cavity and pressing start, the unit will automatically:
- 1. Transfer the fixative from the tank to the process cavity
- 2. Bring the fixative to the selected temperature for the preset time
- 3. Drain, after time expires, the fixative solution back to the tank
- 4. Transfer the decalcifying solution to the cavity and heat it for the selected temperature and time
- 5. Drain the solution back to the tank after completion
Two flushing/rinsing steps are automatically carried out between the two phases. An alarm buzzer advises the operator about the completion of the steps.
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Which rack for which specimens?
Simply position the cassettes in the rack. Position the rack in the cavity and close the cover. Select your protocol and press START. After the decalcification, check the status of the bone.
For specimens of 30x25x5 mm dimensions
For specimens of 30x25x10 mm dimensions
For specimens of 70x50x15 mm dimensions
This is how decalcification should be
All major parameters influencing all these important pre-analytical steps can be controlled, optimized, automated and documented.
Fixation and decalcification temperature can be set between 20-55°C for an optimization of the process.
MAGNETIC STIRRING (AGITATION)
The calcium ions that have been removed can saturate the solution around the specimen. Continuous stirring assures a consistent flow of fresh decalcifier onto the bone surface, accelerating the decalcification process:
SIZE OF SPECIMENS
Three different types of racks are available to fit user’s requirements.
All product surfaces involved in the process circuits are compatible with the following reagents:
|Finefix||EDTA 20% at max 50#C|
|Formalin||Formic acid 20% at max 50°C
Acetic acid 20% at max 50°C
Hydrochloric acid 10% at max 37°C
Nitric acid 10% at max 37°C
Trichloroacetic acid 10% at max 37°C
Decalcification of bone marrow
OPTIMIZING DECALCIFICATION FOR MOLECULAR STUDIES
DECALCIFIER SOLUTION FOR OPTIMAL MOLECULAR STUDIES
For a faster decalcification time, the process with EDTA is best carried out at a pH value of 7.2-7.4. Milestone has developed an innovative, pure EDTA solution, based on a stoichiometric mixture of bibasic and tribasic EDTA to reach this pH value without the addition of an acid/base buffer. The combination of an increased temperature, stirring and the MoL-DECALCIFIER, during the decalcification phase allows fixation, decalcification and processing of bone marrows within 48 hours.
|Formalin 10%||MoL-DECALCIFIER||°C||Fixation + Decal.|
|1h 30’||16h 30’||37||18h|
|Formalin 10%||Formic 10%||°C||Fixation + Decal.|
|1h 30’||1h 30’||37||3h|
DecalMATE protocols for bone marrows (Ø 1.8mm)