Accurate and reliable diagnostic results for morphology and molecular studies
The BoneSTATION consists of two work platforms completed with glass modules for fixation/
The front platform (for the decalcification step) features a heating plate with infrared sensors for
automatic temperature control up to 50°C and magnetic stirring.
The rear platform, for the fixation step, has built-in magnetic stirring only. Two user-friendly work platforms, for easy handling of bone specimens, complete the unit.
The BoneSTATION station can be used with ANY type of fixative/decalcifier, even with strong mineral acids (HCl - HNO3), as all contact surfaces are either glass or PTFE polymer.
The magnetic stirrer assures the solution’s temperature homogeneity.
The PTFE cover condenses vapors, generating a reflux of the reagent for consistent and safe protocols at a constant pH.
A touch-screen terminal allows the user to optimize, standardize and fully document all the processes.
Decalcification of bone marrows
While mineral acids offer a faster decalcification rate, their protocols are more time/temperature sensitive and processed specimens are not optimal for molecular studies. A recent report demonstrates that in this case, recovery of nucleic acids is dramatically reduced.
For this reason, Milestone favors the use of either formic acid or EDTA as “soft” decalcifiers, more suitable for molecular studies. Longer decalcification times are reduced by the optimized agitation and temperature management featured in the BoneSTATION.
|DNA - Yield (ng)||RNA - Yield (ng)||Decal Time|
Note:  Effect of Decalcification Agents on Nucleic Acid Quantity and Quality Veena M Singh et all - USCAP 2012 Poster Presentation
Optimization, Standardization, Documentation
The operator is guided by the touch screen control terminal in setting up standardized and optimized decalcification protocols.
The only manual step of transferring the rack from the fixative container to the decalcifier occurs when an audible alarm advises of the completion of the fixation step. Continuous stirring assures a consistent flow of fresh decalcifier on the bone surface, accelerating the decalcification process by influencing the equilibrium.
Combining agitation with an increased temperature management accelerates the rates of fixation/ decalcification. When both steps are completed, the bone specimens are processed by conventional or rapid microwave technology. Full documentation can be obtained through the provided USB port.
BoneSTATION typical protocols for bone marrows
|Formalin 10%||MOLdecal||°C||Fixation + Decal.|
|1h 30’||16h 30’||37||18h|
|Formalin 10%||Formic 10%||°C||Fixation + Decal.|
|1h 30’||1h 30’||37||2h|
Racks and Reagents
Three acid resistant rack configurations with built-in stirring bar are available to fulfill all specimen sizes.
- Rack for 30 standard cassettes
- Rack for 16 mega cassettes
- Rack for 6 super mega cassettes
Today, standard decalcifying reagents for histological applications are composed of 10% EDTA, and an acid or base is added to bring the solution to the desired pH (7.2-7.4) for the decalcification process.