English languageChinese languageRussian languageSpanish languagePortuguese languageSwedish languagePolish language


The science of cryoembedding
for high-quality frozen sections


The science of cryoembedding for high-quality frozen sections

Need good results?
Start with a good preparation

The optimal frozen section begins with an evenly and completely frozen tissue block in which the block face that will be sectioned is flat and includes a complete, full faced section of the tissue submitted.
The section should be free of ice crystal artifacts, knife lines, and/or tears. The speed and simplicity of PrestoCHILL's operations revolutionize the rapid freezing process, keeping the microscopic appearance of tissues “true-to-life”.

What sets us apart?

The unique combination of the patented face-down embedding technique, together with a state-of-the-art, rapid stirling cooling device at -40°c.

This is how cryoembedding should be!

  • No freezing artifacts
  • 60 seconds freezing time
  • An all-dry system
  • Full downloadable documentation
  • No liquid nitrogen
  • No Co2 - No isopentane

Time to redefine
frozen sections

PrestoCHILL reduces freezing time to 60 seconds: it eliminates freezing artifacts through ultrafast freezing, preventing the formation of ice crystals.
Cut fatty tissues like breast or lymph nodes can be freezed without any sort of difficulty and without the distortion of delicate honeycomb structures.
It is possible to obtain perfectly “flat plane” surfaces to reduce trimming time and to cut easily while using the patented* “face down” embedding technique.
Moreover, it eliminates the compression of artifacts caused by extremely low temperatures and it removes the retraction of tissues: for example, Glomeruli from Bowman’s capsule, and vacuolation spaces around nucleated brain cells, related to formaline fixed, paraffin embedded (FFPE) sections.

Freezing time 60 seconds

Download catalog Ask a question

Simple AND Intuitive operationS make everyone an expert

Step 1

Step 1

Place a drop of the cryo-embedding compound on the tip of the spatula
Step 2

Step 2

Orient the specimen
Step 3

Step 3

Transfer the specimen to the bottom of the mold
Step 4

Step 4

Add the cryo-embedding compound to fill the mold
Step 5

Step 5

Place the chuck into the mold
Step 6

Step 6

Add the extractor and close the cover
Step 7

Step 7

Start the timer. After 60 seconds...
Step 8

Step 8

... a frozen block with a perfectly “flat plane” surface is ready for cutting


The Milestone Application Team has distilled their know-how of several years of research and development of PrestoCHILL into Best Operating Procedures (BOP) for special applications.
They are a step-by-step very easy application guidelines supported also by video tutorials.



How to identify frozen specimens



How to freeze breast and fatty tissues



How to simultaneously freeze small specimens



How to freeze long-thin specimens



How to freeze muscle tissues



How to freeze thin specimens on edge



How to freeze multiple thin specimens on edge



How to freeze skin for MOHS surgery



How to freeze body fluids

Excellent quality with the PRESTO system

Mohs surgery - Cryoembedding of delicate tissues

During the practice of frozen section pathology, pathologists often encounter the task of embedding tissues that are extremely delicate because of the nature of their consistence and structure. This is a situation that occurs daily during Mohs surgery, in which the technologist handles delicate slivers of skin with the goal of accurately embedding these tissues to completely visualize the epidermis and its margin. The PrestoCHILL paper embedding technique offers a safe and rapid solution to these problems.

Two innovative techniques are now available

1 PrestoCHILL paper embedding technique offers a safe and rapid solution to the difficult handling of delicate slivers of skin with the goal of accurately embedding these tissues to fully visualize the epidermis and its margins.

2 PrestoCHILL also offers an alternative technique with rings for those who prefer working with a wide -40C plate when ensuring margins adherence. This technique works seamlessly also with very challenging skin samples.

Take a look at the comparisons of outcomes

Cryoembedding on edge

With the help of a strip of cellulose it is possible to freeze the specimen on edge with the standard freezing procedure as paper disk cryoembedding.

Step 1
Step 2
Step 3

High throughput operations

Each mold can be separately timed through the built-in control panel.
Up to 6 samples can be frozen in parallel or in sequential order in less than 4 minutes.

24/7 operations with fully automatic defrost feature

To eliminate the potential formation of ice on the freezing platform, an automatic defrost cycle is provided. It consists of an embedded heater in the freezing platform and a vacuum pump to extract water vapors from the chamber. Vapors are condensed and collected in a cold-trap placed in the front of the unit for easy handling. A HEPA filter is provided on the exhaust side of the pump. Integrated software can set the defrost cycle to take place during off hours. Afterwards, the cooling cycle automatically restarts at a preset time to ensure the availability for the first case of the day, removing the need to manually monitor the system.

Accessories for easier operation

MCC an optimized cryoembedding compound

MCC an optimized cryoembedding compound

MCC is a proprietary formulation of glycols and resins specifically developed for optimal support during cryotomy of dissected tissues down to -40°C. Being water soluble, MCC doesn’t leave residue on the slides, eliminating non specific background staining. Additionally, MCC will not dull microtome knives.
Lens paper disks for easy mounting

Lens paper disks for easy mounting

Lens paper disks are an excellent support for freezing small fragments or delicated specimens making the diffucult task simple and rapid. Available in 3 sizes (diameters of 16, 22, 30 mm diameters).
Molds to fit every requirement

Molds to fit every requirement

A wide variety of molds with different diameter and depth are available to fullfill every freezing requirement. Since the outside diameter is always the same they can be rapidly exchanged to fit every situation.

Discover our green reagents!



Watch our video to discover how cryoembedding should be for high-quality frozen sections.


Take a moment to consult our catalog to learn all the advantages of Milestone cutting-edge PrestoCHILL for high-quality frozen sections.

Milestone Biobanking Processing

Ask a question

Please type your full name.
Invalid Input
Invalid phone number
Invalid e-mail address
Invalid Input
Invalid Input

You may also be interested in ...


Flash freezing for tissue banking.
English languageChinese languageRussian languageSpanish languagePortuguese language
Swedish languagePolish language