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List of publications based on Milestone instrumentations/reagents.

Vacuum Preservation of Biospecimens - Tissue Safe

  • G. Bussolati, L. Chiusa, A. Cimino and G. D’Armento,
    “Under-Vacuum Preservation of Tissue to be Transferred to Pathology Labs”,
    Department of Biomedical Sciences and Oncology, University of Turin, Turin, Italy.

  • G. Bussolati, L. Chiusa, A. Cimino, G. D’Armento (2007),
    “Tissue transfer to pathology labs: under vacuum is the safe alternative to formalin”,
    Springer-Verlag, 452:229–231
  • A. Sapino, G. D’Armento, L. Molinaro, L. Macrì, A. Cavicchini, G. Bussolati,
    “Gene Expression Profiling in Under-Vacuum Stored Breast Carcinomas: a Pilot Study”,
    Department of Biomedical Sciences and Human Oncology - University of Turin - Italy
  • C. Di Novi, D. Minniti, S. Barbaro, M. G. Zampirolo, A. Cimino, G. Bussolati (2010),
    “Vacuum-based preservation of surgical specimens: An environmentally-safe step towards a formalin-free hospital”,
    Sci Total Environ, doi 10.1016/jscitotenv.2010.04.022
  • A.Moggio, G. D'armento and B. Bussolati,
    “Efficient stel cell isolation from under vacuum preserved tissue samples”,
    Organogenesis 8:3, 1-5 July/August/September 2012
  • G. Bussolati, L. Annaratone and A. Sapino,
    “Vacuum sealing and cooling for safe transfer of tissues from operating theatre to laboratory”,
    Poster, USCAP 2012
  • G.Bussolati,
    “RNA preservation in FFPE tissues requires pre-analytical standardized processing”,
    Poster, USCAP 2011
  • J. F. Flejou, S. El-Naderi, P. Cervera,
    “Vacuum based preservation of colorectal cancer specimens: A comparison with formalin fixation”,
    Medical school Pierre et Marie curie, Paris
  • David Butler et al.,
    “Keeping your Tissue-Safe (Equipment Trial)”,
    Prince Charles Hospital Brisbane Queensland Government Australia RCPA Sydney Australia 2011
  • DP Boyle, R Carson, P Kelly, M Catherwood, S Carroll, L Venkatraman, S McQuaid, H McBride, J James, MB Loughrey,
    “Evaluation of tissue preservation using a vacuum-based refrigeration system for specimen transfer from theatre to laboratori”,
    Department of Pathology, Royal Group of Hospitals Trust, Belfast, Northern Ireland
  • R.Martinez Marin et al.,
    “Traslado de muestras quirurgicas desde quirofano al Servici de Anatomia Patologica. Intraoperatoria “versus” Sistema de alto nivel vacio”,
    BIOBANC – Mur HUVA FFIS IMIB ESBB Congress Tarragona 2010
  • Michael Dib PA (ASCP), Ruan C Varney CT, SSBB, Bruce Jones M.D., Richard J. Zarbo M.D.,
    “Histologic Validation of Vacuum Sealed Formali-Free Tissue Preservation and Transport System”,
    Pathology & Laboratory Medicine Henry Ford Health System, Detroit, Michigan AAPA Congress USA 2012
  • I Marron V.Alvarez et al.,
    “Vacuum Sealing for a safe transfer of tissue from operating theatre to laboratory using TissueSafe Milestone apparaturatus”,
    Hospital Universitario Central De Asturias - Spain ESBB Congress Granada 2012
  • M.Ferronato, B. Filippini, C.Rossetti, A.Scarpa,
    “Vacuum packed transport maintains the RNA quality”,
    ABC-NET Applied Research on Cancer Verona- Italy ESBB Congress Granada 2012
  • M. Caly, A. Léon a, M. Galut b, O. Mariani b, E. Padoy a, X. Sastre-Garau,
    “Analyse qualitative et quantitative d’échantillons tissulaires par le procédé TissueSafe® en vue d’analyses morphologiques et moléculaires. Évaluation sur une série de 10 cas”,
    CRB Centre de Ressources Biologiques Institut Curie. Paris – France Annales de Pathologie Vol 32 - N° 5S
  • B. Poulet, O. Languille, H. Lacluque, G. Hubervic, C. Adem, S. Azoulay, A. Barres,
    “Contrôle de la fixation des mastectomies par la procédure Tissue Safe®”,
    Instutute Pathologie France Annales de Pathologie Vol 32 - N° 5S
  • M. Manzotti, A. Cavallon, M.O. Biasi, T.Tamagni, C. Camia, M. Schiavi, E.Pisa, G. Renne,
    “Evaluation of tissue preservation of material stored at 4°C with TissueSAFE system after the examination and macroscopic sampling”,
    Poster, IEO European Institute of Oncology, Milan

Macro Digital Imaging - MacroPATH

  • F. Joel W-M Leong, A. S-Y Leong (2004),
    “Digital photography in anatomical pathology”,
    J Postgrad Med, 50-1
  • A. S-Y Leong, F. Visinoni, C. Visinoni and J. Milios (2000),
    “An Advanced Digital Image-Capture Computer System for Gross Specimens: a Substitute for Gross Description”,
    Royal College of Pathologists of Australasia, 32. Pp. 131-135


  • A. Leong,
    “Effects of Microwave Fixation and Decalcification in Rodent Tissue”,
    Pfizer Global Research & Development, La Jolla, CA.

Non Formalin Fixation - FineFIX

  • G. Stanta, S. Pozzi Mucelli, F. Petrera, S. Bonin and G. Bussolati (2006),
    “A Novel Fixative Improves Opportunities of Nucleic Acids and Proteomic Analysis in Human Archive’s Tissue”,
    Diagn Mol Pathol, 15-2.
  • A. Gazziero, R. Salmaso, V. GUzzardo, M. Zoletto, R. Salmaso, A. Fassina,
    “Alchoolic Fixative and Quality of Nucleic Acids”,
    Department of Diagnostic Sciences and Special Therapy, University of Padua, Italy.
  • A. Gazziero, V. Guzzardo, E. Aldighieri and A. Fassina (2008),
    “Morphological quality and nucleic acid preservation in cytopathology”,
    Journal of Clinical Pathology.
  • B. Espina, R. Chuaqui, J. Rodriguez, S. Hewitt, C. Ghimenton, F. Visinoni, M. Bellini, C. Compton, J. Vaught; H. Moore, J. Schneider, J. Gillespie,
    “Determination of FineFix fixation on histologic quality ad recovery of RNA from human prostate tissue”.
  • GM Reynolds, BA O’Sullivan, J Junying, PP Taniere, NS Deshmukh (2005),
    “A formalin-free fixative, that provides superior preservation for molecular techniques”,
    Poster Presentation at IBMS meeting – Birmingham, UK

Tissue Processing

Histos KOS Pathos Delta
  • F. Visinoni, J. Milios, A. S.-Y. Leong, M. E. Boon, L. P. Kok, F. Malcangi (1998),
    “Ultra-Rapid Microwave/Variable Pressure-Induced Histoprocessing: Description of a New Tissue Processor”,
    The Journal of Histotechnology / Vol. 21, No. 3
  • Karen Titus (2008),
    “Vision quest – fresh look at AP automation”,
    Cap Today, Vol. 22 No. 8
  • A. S.-Y. Leong and F. J. W-M Leong (2005),
    “Strategies for laboratory cost containment and for pathologist shortage: centralised pathology laboratories with microwave-stimulated histoprocessing and telepathology”,
    Pathology, pp. 1-5
  • A. S.-Y. Leong and David Price (2004),
    “Incorporation of microwave tissue processing into a routine pathology laboratory: impact on turnaround times and laboratory work patterns”,
    Pathology, 36(4), pp. 1-4
  • E. Rossomacha, C. D. Hoemann, A. Chevrier, M. S. Shive,
    “Articular Cartilage and Synovial Tissue”,
    BioSYntech Inc.
  • L. Nava, M. Freschi, C.Doglioni, E. Scapaticci, A. Losa, M. Riva, P. Rigatti, and G. Guazzoni (2007),
    “Definitive histologic diagnosis on prostate biopsies in 3 hours”,
    22st Congress Of The European Association Of Urology, Abstr. 406.
  • K. Alderton, W. Dellow, M. Whitehead, G. Devane,
    “A Microwave Tissue Processor Trial in a Routine Hospital Analtomical Pathology Laboratory”,
    22st Congress Of The European Association Of Urology.
  • Antonio Iesurum, Tiziana Balbi, Domenico Vasapollo, Alberto Cicognani and Claudio Ghimenton (2006),
    “Microwave Processing and Ethanol-Based Fixation in Forensic Pathology”,
    The American Journal of Forensic Medicine and Pathology, 27-2.
  • T. Ragazzini, E. Magrini, M. C. Cucchi, M. Foschini and V. Eusebi (2005),
    “The Fast-Track Biopsy (FTB): Description of Rapid Histology and Immunohistochemistry Methods for Evaluation of Preoperative Breast Core Biopsies”,
    International Journal of Surgical Pathology, 13(3):247-252.
  • Zenobia A. M. Hafajee and Anthony S.-Y. Leong (2004),
    “Ultra-rapid microwave-stimulated tissue processing with a modified protocol incorporating microwave fixation”,
    Pathology, 36(4), pp. 1-5
  • A. M. Mathai, R. Naik, M. R. Pai, S. Rai, P. Baliga (2008),
    “Microwave histoprocessing versus conventional histoprocessing”,
    Indian Journal of Pathology and Microbiology, 51-1, 12-16.
  • Deborah Duckworth (2009),
    “Microwave Processing in the Histology Lab”,
    Pathology at John Hopkins.
  • The Royal Liverpool Hospital (2008),
    “Pathos processing: the Liverpool experience”,
    Pathology in Practise.
  • K. Titus (2008),
    “Vision Quest – Fresh look at AP automation”,
    CAP TODAY, 22-5.
  • E. Parkin, S. Hindocha, D. Subar, I. Mehrban, J. Walls (2010),
    "An Initial Experience with Rapid Microwave Processing in the One-Stop Breast Clinic",
    Socie ́te ́ Internationale de Chirurgie, DOI 10.1007/s00268-010-0747-2.
  • Basavaradhya Sahukar Shruthi, Palani Vinodhkumar, Bina Kashyap, Padala Sridhar Reddy (2013),
    "Use of microwave in diagnostic pathology",
    Journal of Cancer Research and Therapeutics - July-September 2013 - Volume 9 - Issue 3
  • Amanda Bonda, John C. Kinnamona (2013),
    "Microwave processing of gustatory tissues for immunohistochemistry",
    Journal of Neuroscience Methods 215 (2013) 132– 138
  • Joris P. Bulte, Lenny Polman, Margrethe Schlooz-Vries, Annelies Werner, Renate Besselink, Kelly Sessink, Roel Mus, Susanne Lardenoije, Mechli Imhof-Tas, Johan Bulten, Adriana C. H. van Engen-van Grunsven, Ewout Schaafsma, Luc J. A. Strobbe, Peter Bult, Johannes H. W. De Wilt (2012),
    "One-day core needle biopsy in a breast clinic: 4 years experience",
    Breast Cancer Res Treat DOI 10.1007/s10549-012-2372-2
  • Lynne Doverty and Linda Davidson (2013),
    "Evaluation of Xylene-free Tissue Processing on the LOGOS all-in-one Hybrid Tissue Processor",
    Pathology Department Aberdeen Royal Infirmary, Poster
  • Linda Graham (2012),
    "Microwave Processing for Electron Microscopy",
    Anatomical Pathology, LabPlus. Poster
  • Richard J Zarbo, Ruan C Varney, Michael J Dib, Beverly Mahar and Jason Wozniak (2012),
    "Validation of Histology Tissue Processing and Stain Quality of Logos Rapid−Cycle Microwave Processor in Lean Continuous Flow Operations",
    Department of Pathology, Henry Ford Health System, Detroit, Michigan, Poster
  • RJ Zarbo, NS Gupta, R Varney, N Main, S Richard, B Mahar, ON Kryvenko, S McMahon, N Draga, O Alassi, A Ormsby, R D'Angelo (2011),
    "Optimization of fixation and processing of biopsy gun prostate needle biopsy specimens",
    Department of Pathology, Henry Ford Health System, Detroit, Michigan, Poster

Antigen Retrieval

  • A. S-Y Leong, E. Lee, Z. Haffajee (2002),
    “Superheating Antigen Retrieval”,
    Applied Immunohistochemistry & Molecular Morphology, 10 - 3 - pp. 263-268
  • A. S-Y Leong, Z. Haffajee, M. Clarke (2007),
    “Microwave Enhancement of CISH for HER2 Oncogene”,
    Applied Immunohistochemistry & Molecular Morphology, 15 -1 - pp. 88-93

Electron Microscopy Process of Specimens - REM

  • J. A. Shröder (2008),
    “Clinical Electron Microscopy”,
    G.I.T. Imaging & Microscopy, 2/2008, pp 32–35.
  • J.A. Schroeder, H.R. Gelderblom, B. Hauroeder, Ch. Schmetz, J. Milios and F. Hofstaedter (2004),
    “Same-Day EM-Diagnosis of Tissues and Potential Bioterrorism Samples by the Use of Microwave-Assisted Processing”,
    Microsc Microanal, Microscopy Society of America, 10(Suppl 2).
  • J. A. Schroeder, H. R. Gelderblom, B. Hauroeder, C. Schmetz, J. Milios, F. Hofstaedter (2005),
    “Microwave-assisted tissue processing for same-day EM-diagnosis of potential bioterrorism and clinical samples”,
    Elsevier Ltd., Micron 37, pp. 577-590.

Fixation - Formalin Fixation Gross Hardening

  • Ciara Barrett, Francesca Brett, David Grehan, and Micheal B. McDermott (2004),
    “Heat-Accelerated Fixation and Rapid Dissection of the Pediatric Brain at Autopsy: A Pragmatic Approach to the Difficulties of Organ Retention”,
    Pediatric and Development Pathology 7, 595-600.
  • RJ Zarbo, NS Gupta, R Varney, N Main, S Richard, B Mahar, ON Kryvenko, S McMahon, N Draga, O Alassi, A Ormsby, R D'Angelo (2011),
    "Optimization of fixation and processing of biopsy gun prostate needle biopsy specimens",
    Department of Pathology, Henry Ford Health System, Detroit, Michigan, Poster